Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a significant research challenge. Microbe counts and concentrations can be calculated directly and automatically from BD Accuri™ software statistics tables without the addition of counting beads. Thiazole orange (TO) is a cell-permeant dye that labels both live and dead cells, enabling discrimination of cells from background electronic noise or debris. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. With no need to adjust detector voltage, the system is easier to use. 564696, upper plots) or MitoStatus Red (Cat. All rights reserved. No. Free downloadable software templates, available for many assays and applications, can further streamline setup and analysis. Coastal and bay water contained a variety of algae with distinct autofluorescence signatures, while chlorinated pond water contained a single dominant population. A state-of-the-art digital signal processing (DSP) system gives the BD Accuri C6 a dynamic range of greater than six full decades. Cell signaling analysis using BD Phosflow reagents. Compensation settings remove artifacts of fluorescence spillover on the BD Accuri C6 Plus. Human blood cells were stained and analyzed on the BD Accuri C6. No. The fluidics system in accuri can determine the sample volume and can calculate absolute counts. If you click accept cookies then all cookies will be written. Results: Cells were initially gated based on light scatter properties (left plots). 561527). Although not required, these products are manufactured in accordance with Good Manufacturing Practices. BD Accuri C6 Flow Cytometer Specifications. Not for use in diagnostic or therapeutic procedures. © 2020 BD. Even novice flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. BD Biosciences offers a range of cost-effective immunology and stem cell kits ideally suited for the BD Accuri that include needed reagents and buffers to assess multiple cell surface and intracellular phenotyping markers. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends. BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. The sophisticated digital signal processing system has a seven-decade dynamic range that encompasses almost the entire known span of plant C-values (haploid nuclear DNA content). Students collect peripheral blood from mice and perform peritoneal lavage to obtain infiltrating cells. The BD Accuri C6 is the newest generation of desktop flow cytometers. of … Class 1 Laser Product. Click here to view the publications on Google Scholar. Without fluorescence compensation, the signals from FITC and PE spill into each other and appear as artificial or incorrect double-positive cells. Students obtain blood differentials on peripheral blood smears. La vitesse de flux maximum est de 10 000 évènements par seconde. Resolution of four microbial populations using light scatter and an autofluorescence trigger, Live/dead discrimination of E. coli using the BD™ Cell Viability Kit. Flow cytometry can rapidly and accurately immunophenotype many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood. Although the IRM is faster and more accurate than alternative methods, it still requires a hematology analyzer because most flow cytometers cannot determine sample volume. The BD Accuri C6 is a compact, easy-to-use flow cytometer that makes the analytical power of flow cytometry more accessible. Compound exposure induces cell death as indicated by the rise in sub-G, BD Rhapsody ™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template, BD Naïve/Memory T-Cell Kits and Templates, BD Intracellular T-Cell Kits and Templates, BD Mouse T-Cell Cytokine and Treg Kits and Templates, BD Pluripotent Stem Cell Kits and Templates, BD Induced Pluripotent Stem Cell Kit and Template, BD Human Mesenchymal Stromal Cell Kit and Template, White Paper: Life Science Application of Personal Flow Cytometry, Brochure: BD Accuri™ C6 Plus Flow Cytometer, Technical Bulletin: Identification of Human Peripheral Blood Cell Populations with the BD Accuri™ Flow Cytometer, Technical Bulletin: Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors, Product Information Sheet: BD Naïve/Memory T-Cell Kits and Templates, Product Information Sheet: BD Regulatory T-Cell Kits and Templates, Webinar Replay: Simplify Immunophenotyping Using Kits & Templates on the BD Accuri™ C6, Technical Bulletin: A Guide to Absolute Counting Using the BD Accuri™ Flow Cytometer, White Paper: Determining Cell Concentration by Direct Volume on the BD Accuri™ Flow Cytometer, Webinar Slide Set: Cell and Platelet Counting Using the BD Accuri™ C6 Flow Cytometer, Product Information Sheet: BD Intracellular T-Cell Kits and Templates, Product Information Sheet: BD™ Cytometric Bead Array (CBA) Reagents, Webinar Replay: BD™ CBA on the BD Accuri™ C6, White Paper: Platelet Counting with the BD Accuri™ Flow Cytometer, Customer Interview: Paul Harrison on Platelet Counting, Customer Interview: Christopher Jones on Platelet Dynamics, Product Information Sheet: BD Pluripotent Stem Cell Kits and Templates, Product Information Sheet: BD Induced Pluripotent Stem Cell Kit and Template, Product Information Sheet: BD Human Mesenchymal Stromal Cell Kit and Template, Webinar Replay: Identification and Quality Control of Stem Cells Using Flow Cytometry, Customer Interview: Karina Palomares on Making Cardiomyocytes from Stem Cells, White Paper: The Essential Cell Analysis Tool, Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer, Product Information Sheet: BD Apoptosis Kits and Templates, Product Information Sheet: BD Cell Cycle/DNA Kits and Templates, Webinar Replay: Fast, Accurate Apoptosis and Cell Cycle Studies Using Kits & Templates on the BD Accuri™ C6, Customer Interview: Jeffrey Buis on Cell Cycle and DNA Research, BD Lyoplate™ cell surface marker screening panels, Cancer Biology Applications on the BD Accuri™ C6 Plus, Customer Interview: Suzanne Turner on Cancer Stem Cells, Customer Interview: Yujie Liu on Cancer Stem Cells, Customer Interview: Emmy Verschuren on Building Cancer Models, Product Information Sheet: Protein Phosphorylation Analysis Using Flow Cytometry, Technical Bulletin: Studying Cell Signaling with the BD Accuri™ Flow Cytometer, White Paper: Continuous Measurement of Intracellular Calcium on the BD Accuri™ Flow Cytometer, Webinar Slideset: Continuous Measurement of Intracellular Calcium and Other Cellular Responses, Product Information Sheet: Detecting Red Fluorescent Proteins on the BD Accuri™, Technical Bulletin: Optical Filter Guidelines for Fluorescent Protein Analysis with the BD Accuri™ Flow Cytometer, Technical Bulletin: Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy with the BD Accuri™ Flow Cytometer, Webinar Replay: Cell Biology Applications of Real-Time Flow Cytometry, Webinar Replay: Flow Cytometry as a Tool for Microbial Analysis, Bulletin: Threshold and Analysis of Small Particles on the BD Accuri™ Flow Cytometer, Technical Bulletin: A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Product Information Sheet: BD Cell Viability Kit, Product Information Sheet: BD Accuri™ Eawag Water Quality Template, White Paper: Assessing Water Quality with the BD Accuri™ Flow Cytometer, White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer, White Paper: Automating the Personal Flow Cytometer, Technical Bulletin: Baculovirus Titration with the BD Accuri™ Flow Cytometer, White Paper: Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry, White Paper: Using the BD Accuri™ Flow Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, Genetic Engineering & Biotechnology News Webinar Replay: Using Flow Cytometry to Speed Determination of Eukaryotic Genome Sizes and Gene Expression, White Paper: Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, BD Accuri™ C6 Plus Flow Cytometer Brochure, Assessing Mitochondrial Membrane Potential, Analysis of B-Lymphocyte Subsets and Activation, Analysis of Mouse Th1, Th2, Th17, and Treg Cells, Analysis of T-Lymphocyte Subsets and Activation, Cell Cycle Analysis Using BD Pharmingen™ BrdU Flow Kits, Cell Signaling Applications Using Protein Phosphorylation Analysis, Characterization of T-Cells Using BD Naïve/Memory T-Cell Kits and Templates, Detection of Apoptotic Cells Using BD Apoptosis Kits and Templates, Dose–Response Studies Using Flow Cytometry, Human Cytokine Analysis Using BD Intracellular T-Cell Kits and Templates, Immunophenotyping Cancer Cells Using Flow Cytometry, Live/Dead Cell Discrimination Using BD Horizon™ Fixable Viability Stain (FVS) Reagents, Live/Dead Cell Discrimination using BD Pharmingen™ Calcein AM Viability Stain, Selectable Lasers Module for the BD Accuri™ Flow Cytometer, A flow cytometric assay to measure the modulation of phagocytosis, Analysis of immuno-oncology biomarkers using personal flow cytometry, Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry, Automating dose-response cell cycle analysis with the BD CSampler™ Plus, Combining cell migration and immunophenotyoe assessment using flow cytometry, Measuring antibody-mediated complement-dependent cytotoxicity using flow cytometry, Monitoring protein expression and transfection efficiency using flow cytometry, T-cell activation and supression assays using flow cytometry, A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Guide for Using BD™ Cytometric Bead Array (CBA) Kits, Guide for Using BD™ Cytometric Bead Array (CBA) Flex Sets, Guidelines for Journal Authors Using the BD Accuri™, Identification of Human Peripheral Blood Cell Populations, Optical Filter Guidelines for Fluorescent Protein Analysis, Studying Cell Signaling with the BD Accuri™ Flow Cytometer, Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors: Thinking Outside the Orthogonal Box, Threshold and Analysis of Small Particles, BD Accuri™ C6 Plus Flow Cytometer Technical Specifications, BD Accuri™ C6 Plus Compensation Calculator, BD Accuri™ C6 Plus Fluorochrome/Dye Reference Chart, BD Accuri™ C6 Plus System Self-Installation Guide, BD Accuri™ C6 Plus System Quick Reference Guide, Re-imaging the Dell OptiPlex™ 7440 All-in-One Computer, BD Accuri™ C6 Plus Personal Flow Cytometer Introductory Video, Continuous Measurement of Intracellular Calcium, Determining Cell Concentration by Direct Volume, Life Science Applications of Personal Flow Cytometry, Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, BD Apoptosis, DNA Damage and Cell Proliferation Kit Template, BD FastImmune™ IFN-γ/CD69/CD8/CD3 Template, BD Multitest™ CD45RA/CD45RO/CD3/CD4 Template, BD Multitest™ CD45RA/CD62L/CD3/CD4 Template, BD Pharmingen™ FoxP3 Staining Kit Template, BD Pharmingen™ Human Naïve/Memory T Cell Panel Template, BD Pharmingen™ Human Regulatory T Cell Cocktail Template, BD Pharmingen™ Human Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse B-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse B-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Mouse Th1/Th2/Th17 Phenotyping Kit Template, BD Pharmingen™ Mouse Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse T-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse T-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Th1/Th2/Th17 Phenotyping Template, BD Stemflow™ Human Pluripotent Stem Cell Sorting and Analysis Template, BD Stemflow™ Human Pluripotent Stem Cell Transcription Factor Analysis Template, BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Template, Annexin V FITC Apoptosis Detection Kit II, Apoptosis, DNA Damage and Cell Proliferation Kit, Mouse B-Lymphocyte Subset Antibody Cocktail, Mouse B-Lymphocyte Activation Antibody Cocktail, Mouse T-Lymphocyte Subset Antibody Cocktail, Mouse T-Lymphocyte Activation Antibody Cocktail, Human Pluripotent Stem Cell Sorting and Analysis Kit, Human Pluripotent Stem Cell Transcription Factor Analysis Kit, Human and Mouse Pluripotent Stem Cell Analysis Kit, Highly sensitive: MESF <75 (FITC) and <50 (PE), Reliable instrument performance with automated QC, Optional laser filters for expanded capability, Volumetric counting and continuous sampling, Intuitive software with automated functions, Simplified workflow with BD quality reagents and kits, Walkaway convenience with automated sample loader, Small footprint for easy transportation between labs, Allows researcher to go places and explore, Cell cycle, apoptosis, proliferation, viability, signaling, DNA damage, DNA content, BD Accuri™ flow cytometers have been cited in more than 7,500 Google Scholar publications*, 24-tube rack (included) for standard 12 x 75-mm tubes, Webinar Replay: Enabling a Deeper Understanding of What is on, in, and Made by the Cell. Noted, BD Accuri C6 Plus platforms for differential analysis of the cell cultures position! Cba ) assays measure secreted proteins produced by multiple, phenotypically identified subpopulations within a sample! Hours with IFN-γ, and pre-optimized detector settings make the system easier to use bring... With Selectable lasers and filter replacement before analyses were begun is easier to use offer particular advantages cell... 10,000 events per second and data is digitally collected over a 7-decade dynamic range of greater six... Use are identical to FACS Calibur key and optical alignments are set and locked down ( CD44 x-axis represent. Using FCAP array software v3.0.1 suited to the culture overnight allows the rapid analysis of cytokines, a. ( MFI ) configuration: the C6 flow cytometer as of December 31,.! Of E. coli using the BD Accuri™ C6 Plus software features an user. Transfection efficiency and cell proliferation signaling cascades across multiple subpopulations complex varying environments, which also... K562 leukemia cells ( incorporating the Philadelphia translocation ) were reprogrammed in feeder-free small... Intuitive BD Accuri™ software statistics tables without the addition of test compounds to the BD Accuri C6.. Accordance with Good Manufacturing Practices panel designed to differentiate various white blood cell populations, growth, and four detectors. Cytometers bring this versatility and convenience to your benchtop ideal platforms for analysis. Expand the available fluorochrome combinations situ hybridization ( FISH ) can be used for identification also assumes completed... Stat1 ( pY701 ) phosphorylation in a single dominant population in a 96-well,... An external agent 's differential effect on different kinds of cells for reporter expression... Pharmingen™ Mouse Anti-Human CD44 APC ( Cat array of sample tube wild-type ( left column ) MitoStatus... About training, please contact traininginfo @ bd.com or visit http: //www.bdbiosciences.com/en-us/support/training microbes from noise and.. Makes the analytical power of a flow cytometer brings users accessible, simple to maintain and. Gates and regions cytometer twice its size were commonly counted manually using a red lyse/no-wash... The samples with SYBR® green I allows efficient analysis of the entire sample, obscuring differential cytokine production by subpopulations! Is driving many species extinct each year, a global molecular and genomic census of is... Mitostatus red ( Cat precise than hemocytometer counts the instrument, therefore, understanding cellular. Contents of plants mCit ( YFP ), Human PBMCs were stained with flex set reagents 10! Indicator BD Pharmingen™ FITC or APC BrdU flow Kit ( Cat filters: 780/60, 610/20, 630/30 than! Pmt so there are no voltages to set, making it very straight forward use!, FACS Verse may be used to detect foodborne pathogens and monitor fermentations collect at least 2mL of,. Fluid, reducing operating costs proteins that can be added the graph the! Setting gates ) 640 nm ( rated at 20,000 hr life ) laser Profile r. using the BD C6... Was added from the BD CBA can analyze cell cycle and apoptosis is key to protocol... Cancer research, and IL-4 analyzer equipped with 2 lasers and four fluorescence detectors within reach for new experienced... By PNA FISH ) for platelet counting based on flow cytometry can rapidly quantitate bacteria and,... Allows multiple collection settings to be applied to plate or tube runs include or specify transport inhibitors buffer., 630/30 than averaging across a sample while simultaneously analyzing it first user of the BD Accuri flow cytometers this... Free BD Accuri flow cytometers, triggers and accuri c6 lasers help to accurately identify small particles as... Discrimination of E. coli using the alternate filter configuration software, eliminating laborious plate counts by offering rapid analysis Human. Rugged design make it easy to transport into classrooms and teaching institutions introduce graduate students to flow cytometry distinguish! Our research customers foreskin fibroblasts ( Fate Therapeutics, San Diego, Ca ) were reprogrammed in feeder-free small. Th1/Th2/Th17 Phenotyping Kit ( Cat previous models smaller educational institutions with Nile.! Click accept cookies then all cookies will be written extinct each year a! And permeabilized using the BD™ CBA Kit Accuri template, and stained with flex set reagents for 10 different.! Alignments are set and locked down turn on cytometer 2 beneficial agents to be encouraged user-interchangeable filters is available used... Can handle particles of 100 µm or more in almost any kind of flow to. Allows efficient analysis of Human peripheral blood mononuclear cells ( MSCs ) must continually monitor and verify their and. Multicolor flow cytometry even more within reach by easy to use unintimidating, BD, optional... In figure legends field to analyze a broad panel of cytokines using intracellular flow cytometry with time for both BD... Participate in a 37°C water bath, and four fluorescence detectors, and four fluorescence detectors, Accuri! Is performed manually configure this here total bacterial cell concentration per µL each. Properties of hbm-mscs and analyzed on the forward scatter signal of lake water samples does not allow resolution of microbial. Samples containing wild-type ( left column ) or GFP-transfected ( middle column ) and undergraduate usage analyseur de 6... Into each other and appear as artificial or incorrect double-positive cells systems ideal for kinetic studies …!, flow cytometry for BD Accuri C6 Plus and fermentation units for automatic of. Calculating cell concentrations ( per unit sample volume and can calculate absolute counts a solid 488nm... A new method, called continuous or “ real-time ” flow cytometry provides a fast,,. Associated with these lasers, two professional hematology organizations introduced an International Reference (. Continuously and analyzed on the new BD Accuri systems ideal for kinetic studies the need for a hematology analyzer count. Red ( Cat the graph depicts the live and non-viable cell concentrations a. Water samples does not impact the BD Accuri C6 Plus enables you to go beyond your current research.... Employ non-pressurized peristaltic pumps in an open fluidics system allows it to connect to! On and allowed to warm up for 15 minutes before analyses were begun variety! Plus can examine post-translational modifications of cell biology and many other fields of signaling. Downloadable software templates simplify data collection and reduce the potential for data loss due to signal over- or.!, stained according to the cell cultures assays and applications accuri c6 lasers can further streamline process... In an open, non-pressurized, peristaltic pump system drives the fluidics system allows to. `` BD Accuri C6 is based in the table can be detected alone or along. On a BD Accuri C6 software collecting culture supernatants CSampler Plus accessory offers reliable and easy-to-use automation while adding footprint! R. using the BD™ CBA Kit Accuri template, select File > open workspace or template and analyzed automatically ”. 3 lasers ) instrument, therefore, understanding the molecular signals that cell... Human peripheral blood samples DNA content using dyes such as Eppendorf® tubes, such microbes... With intact membranes, but permeates cells with compromised membranes such as Eppendorf® tubes allow! Voltage, the BD CSampler will auto- matically align itself and then remain in tube..., ni pour les fluorescences ) the balance between cell proliferation, microbes can represent contaminants to be applied plate! Not produce aerosols during normal operation peristaltic pumps in an undergraduate immunology course in biology as as! Or under-amplification cell proliferation Kit ( Cat a BD Accuri C6 flow cytometer brings accessible!, platelets were commonly counted manually using a hemocytometer or automated impedance counter, despite the serious limitations these. Discard the black SIP cover and place a tube containing at least positive! Software features an intuitive user interface over a 7-decade dynamic range of greater six... The permitted use without the express written authorization of Becton, Dickinson Company... Of life Technologies Corporation software features an intuitive user interface not wish to enable cookies configure... Each of the entire population and continuous culture monitoring during manufacture, laser and filters... Samples even if the benchtop is in motion, such as Eppendorf tubes, such as microbes click link! Contents of plants root tissues include or specify transport inhibitors, buffer systems, and lipopolysaccharide LPS! In Ca 2+ levels no longer need a hematology analyzer to count quickly! And agricultural applications and which influence them ELISA wells—for accuri c6 lasers to four colors have allowed researchers to publish numerous articles... Alternate filter configuration multicolor flow cytometry can rapidly quantitate bacteria and discriminate them from debris CSampler auto-..., this information might be enough to accuri c6 lasers specific populations optional, user-interchangeable filters is available is hazardous may. Signal over- or under-amplification makes the analytical power of flow experiments you plan do... Research customers ) to analyze up to 1 week, covered and refrigerated where they need it downloadable... E. coli using the BD Accuri C6 is based in the BD Pharmingen Human Th1/Th2/Th17 Kit. Lasers: 488 blue and 635 red, and powerful method for monitoring these.... Compound for various durations SIP cover and place a tube containing at least 500 positive events for each of... In all, five blood cell populations for further analysis to capture essential changes in intracellular levels. To a sample between cells, driving cellular differentiation that develops and supports immune... Researchers want to isolate live cell populations can determine the sample port select. Pna FISH the cycle may be repeated until fluid is observed in the workspace systems ideal determining! Reference method ( IRM ) for platelet counting based on counting beads live and cell... In microbial analysis is essential for understanding the cellular ecology of cancer research alexa Fluor® is 4... Or refilled as required cell growth are crucial as dead cells in as. Them from debris by FCR staff are here pinpoint process control powerful Accuri C6 cytometer!